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Validation of pH Standards and Estimation of the Activity ...

We selected and validated the pH values of three standard materials that function in the protic ionic liquid, ethylammonium nitrate (EAN). The pH values of 0.05 mol kg–1 phthalate, oxalate, and phosphate buffers were 4.93 (0.04), 2.12 (0.04), and 7.13 (0.06), respectively (the values in the parentheses denote the standard deviation). Because the pH of EAN ranges from 0 to 10, with a neutral ...

Author: Ryo Kanzaki, Hikaru Daiba, Hitoshi Kodamatani, Takashi Tomiyasu

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Excised organs were placed in 1.7 mL Eppendorf tubes containing 0.5 mL 2.5 % glutaraldehyde buffered with cacodylate-sucrose solution (0.1 mol L −1 sodium cacodylate and 0.1 mol L −1 sucrose, pH 7.6) and stored at 4 °C until processing (see section 2.8). The remaining 3 pairs were euthanized and fixed in formalin as below and allocated for ...

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Using a pH Meter | Protocol

Cite this Video. JoVE Science Education Database. General Chemistry. Using a pH Meter. JoVE, Cambridge, MA, (2020). Principles. The pH of a solution may be accurately and easily determined by electrochemical measurements with a device known as a pH meter with a pH (proton)-sensitive electrode (usually glass) and a reference electrode (usually silver chloride or calomel).

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Real-time assay for exosome membrane fusion with an ...

A 5 μL aliquot of a 10 μg/mL anti-CD63 solution was added to either the cationic SLB membrane (EPC:PC:chol = 1:1:1, mol/mol/mol) or the neutral SLB membrane (PC:chol = 2:1, mol/mol) and fused with a defined number of HEK-293 exosome particles. The conductance values were recorded after exosome fusion at an applied voltage +100 mV.

3.2 TEST FOR STERILITY - World Health Organization

pH after sterilization 7.1 to 7.5. Dissolve the solids in water R, warming slightly to effect solution. Cool the solution to room temperature. Add sodium hydroxide (1 mol/l) VS, if necessary, so that after sterilization the solution will have a pH of 7.1 to 7.5. Filter, if necessary, to clarify, distribute into suitable

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What Is the Difference Between Molarity and Normality?

Because normality references concentration with respect to the reactive species, it's an ambiguous unit of concentration (unlike molarity). An example of how this can work may be seen with iron(III) thiosulfate, Fe 2 (S 2 O 3) 3.The normality depends on which part of the redox reaction you're examining.

Author: Todd Helmenstine

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Validation of pH Standards and Estimation of the Activity ...

We selected and validated the pH values of three standard materials that function in the protic ionic liquid, ethylammonium nitrate (EAN). The pH values of 0.05 mol kg–1 phthalate, oxalate, and phosphate buffers were 4.93 (0.04), 2.12 (0.04), and 7.13 (0.06), respectively (the values in the parentheses denote the standard deviation). Because the pH of EAN ranges from 0 to 10, with a neutral ...

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Excised organs were placed in 1.7 mL Eppendorf tubes containing 0.5 mL 2.5 % glutaraldehyde buffered with cacodylate-sucrose solution (0.1 mol L −1 sodium cacodylate and 0.1 mol L −1 sucrose, pH 7.6) and stored at 4 °C until processing (see section 2.8). The remaining 3 pairs were euthanized and fixed in formalin as below and allocated for ...

3.2 TEST FOR STERILITY - World Health Organization

pH after sterilization 7.1 to 7.5. Dissolve the solids in water R, warming slightly to effect solution. Cool the solution to room temperature. Add sodium hydroxide (1 mol/l) VS, if necessary, so that after sterilization the solution will have a pH of 7.1 to 7.5. Filter, if necessary, to clarify, distribute into suitable

Analytical Sciences - JST

The response times for a 95% signal change (t 95%) were calculated to be 27 ± 2 s (n = 5) for a sample change from pH 4.0 to 1.0, and 23 ± 2 s (n = 5) for reverse change from pH 1.0 to 4.0. These results indicate that the novel pH optode allows for accurate and rapid measurements of pH values.

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Real-time assay for exosome membrane fusion with an ...

A 5 μL aliquot of a 10 μg/mL anti-CD63 solution was added to either the cationic SLB membrane (EPC:PC:chol = 1:1:1, mol/mol/mol) or the neutral SLB membrane (PC:chol = 2:1, mol/mol) and fused with a defined number of HEK-293 exosome particles. The conductance values were recorded after exosome fusion at an applied voltage +100 mV.

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